mRNA analysis in different mouse tissues

The functioning of a specific tissue depends on the expression pattern of the different genes. We used microarrays to compare gene expression across different murine tissues, to get a better understanding in the expression pattern and functioning of the different tissues. With this analysis, we were not only able to identify genes that were specifically expressed in a specific tissue but, as important, we also identified genes that were specifically repressed in a tissue, compared to al the other analysed tissues. C57Bl6/j6 (Janvier) mice between 10 and 12 weeks old were used to isolate 22 different tissues. Mainly male mice were used, except for placenta and ovary, where female mice were used. Also the mouse insulinoma cellline MIN6 was analysed in this study. For every tissue, at least 3 biological replicates were used.特定组织的生理功能依赖于不同基因的表达模式。本研究采用微阵列（microarrays）技术，对不同小鼠组织的基因表达水平进行对比分析，以期更深入地解析各类组织的基因表达模式与生理功能。通过本次分析，我们不仅成功鉴定出仅在特定组织中特异性表达的基因，尤为重要的是，相较于其余所有已分析的组织，我们还鉴定出了在某一组织中特异性表达受抑制的基因。本研究选取10至12周龄的C57Bl6/j6（Janvier）品系小鼠，从中分离得到22种不同的组织。实验主要使用雄性小鼠，仅胎盘与卵巢组织采用雌性小鼠。本研究同时对小鼠胰岛素瘤细胞系MIN6进行了分析。对于每一种组织样本，我们均设置至少3次生物学重复。