Comparison of Nine Extraction Methods for Bacterial Identification Using the ONT MinION Sequencer

The purpose of this study was to identify a universal extraction method that could be coupled with ONT MinION sequencing for use in forensic situations for rapid identification of bacteria using the 16S FASTQ pipeline. It also evaluated the cloud-based data analysis software that is provided by ONT, EPI2ME. Eight test bacteria and nine extraction methods were tested.