RNA-sequencing of Early vs Late Mouse Pancreas Reentrainment

The objectives of this study was to understand the implication of early (day 1:ZT8) vs late (day 8 [for female]/day 9 [for males]:ZT176/200) reentrainment in mouse pancreas following a chronic (1-month) phase shift protocol. 4-6 week old mice underwent 4 weeks of either a normal circadian light-dark cycle or a circadian disrupted light-dark cycle whereby mice were phase shifted forward 8 hours every 2-3 days. At 4 weeks all mice were normalized to normal circadian conditions 12 hours prior to the 4 week mark. Mice were then sacraficed at 14:00 each day. Of the mice evaluated, those from ZT8 and ZT176/200 were selected for further evaluation with RNA-sequencing. A total of 24 samples were included from two timepoints. At each timepoint, 6 mice were evaluated from each condition, including normal circadian and circadian disrupted conditions. A total of 3 males/females were included per condition per timepoint.本研究旨在明确慢性（1个月）节律移位方案实施后，小鼠胰腺内早期（第1天：授时因子时间8，ZT8）与晚期（雌性第8天/雄性第9天：ZT176/200）再同步的生物学效应。选取4~6周龄小鼠，随机分为两组：一组维持正常昼夜光照周期，另一组接受每2~3天向前相位移位8小时的节律紊乱光照周期，造模周期为4周。造模第4周时，所有小鼠均恢复至正常昼夜节律环境，并于该时间点前12小时完成节律适应。随后每日14:00对各组小鼠实施安乐死。在受试小鼠中，选取来自ZT8与ZT176/200两个时间点的样本开展RNA测序（RNA-sequencing）进一步分析。本研究共纳入24个样本，覆盖两个时间点；每个时间点下，正常昼夜节律组与节律紊乱组各纳入6只小鼠，且每组每时间点均包含3只雄性与3只雌性小鼠。