Disruption of the ATXN1-CIC complex causes a spectrum of neurobehavioral phenotypes in mice and humans

We isolated RNAs from Otp-lineage cells in the mouse brain using translating ribosome affinity purification (TRAP) approach. We compared two groups of mice: Otp-cre; ROSAfsTRAP (control) and Otp-cre; Cicf/f; ROSAfsTRAP (conditional knockout). Following RNA isolation, RNAseq was performed and gene expression profiles were compared between controls and conditional knockouts. Examination of gene expression in Otp-lineage neurons in two mouse groups.我们采用翻译核糖体亲和纯化（translating ribosome affinity purification, TRAP）技术，从小鼠大脑的Otp谱系细胞中分离提取核糖核酸（RNA）。本研究设置两组对照小鼠：Otp-cre; ROSAfsTRAP（对照组）与Otp-cre; Cicf/f; ROSAfsTRAP（条件性基因敲除组）。完成RNA分离后，我们开展了RNA测序实验，并对对照组与条件性基因敲除组的基因表达谱进行对比分析，以考察两组小鼠Otp谱系神经元的基因表达特征。