Epigenetic analysis of BlaER1 transdifferentiation. Epigenetic analysis of BlaER1 transdifferentiation

Study of BLaER1 cell line epigenetic changes induced throughout transdifferentiation. The Illumina Infinium MethylationEPIC Beadchip was used to obtain genomewide methylation profiles of BLaER1 cells at 7 different times throughout transdifferentiation treatment (0h, 3h, 12h, 24h, 48h, 72h and 168h). As a reference, the parental RCH-ACV cell line at 168h of treatment and anonymous donor blood derived macrophages were also profiled. Overall design: Bisulphite converted DNA from BLaER1 at seven time points of transdifferentiation treatment (0h, 3h, 12h, 24h, 48h, 72h and 168h), RCH-ACV and macrophages, were hybridised to the Illumina Infinium MethylationEPIC Beadchip.