GNPS - Eelgrass rhizosphere exometabolites Exp1 LC-MS2 NEG

This project was conducted in collaboration with the Stachowicz and Dorrestein labs exploring the effects of eelgrass genotypic diversity on rhizosphere exometabolome production under marine heat wave versus ambient conditions. This data was collected from cultures of eelgrass genotypes grown in various levels of poly or monoculture under marine heat wave or ambient conditions in mesocosm. After simulating a 15 week marine heat wave regime, we sampled for each culture type-treatment combination’s rhizosphere metabolome and used non-targeted LC-MS/MS to examine how root exudates at the culture-level interact with genotypic diversity and temperature stress in a marine habitat forming species. Untargeted LC-MS/MS acquisition was performed on a Vanquish Flex UHPLC system coupled to an Orbitrap Exploris 240 (Thermo Fisher Scientific, Bremen, Germany). Chromatographic separation was performed on a Kinetex 1.7 um 100 A pore size C18 reversed phase UHPLC column 150 x 2.1 mm (Phenomenex, Torrance, CA).