Evaluation of the roles of Lats1 and Lats2 in the development of the Mullerian ducts

Development of the female tract results from the careful coordination of numerous signaling pathways. Here, we evaluated the role of hippo pathway in the development of the female reproductive tract. We used microarray to compare the gene expression changes observed in the Mullerian ducts of E17.5 mice embryos between control group and experimental (mutant) group bearing a conditional deletion of both (conjoint) Lats1 and Lats2 using the Amhr2cre strain. Mullerian ducts were collected from E17.5 mice (control and experimental group) for RNA extraction and hybridization on Affymetrix microarrays. Control=Lats1f/f,Lats2f/f , Mutant=Lats1f/f,Lats2f/f,Amhr2cre/+雌性生殖道的发育源于多条信号通路的精密协同调控。本研究探究了Hippo通路（Hippo pathway）在雌性生殖道发育中的作用。本研究采用基因芯片技术，对比了对照组与实验组（突变组）E17.5天小鼠胚胎米勒管（Müllerian ducts）中的基因表达变化：实验组通过Amhr2cre工具株实现了Lats1与Lats2的联合条件性敲除。研究人员从E17.5天的对照组与实验组小鼠体内分离米勒管，用于RNA提取及Affymetrix基因芯片杂交实验。对照组基因型为Lats1f/f、Lats2f/f，突变组基因型为Lats1f/f、Lats2f/f、Amhr2cre/+