Estrogen receptor alpha (ESR1) gene amplification is frequent in breast cancer. Subsequent Supplementary Optical Dataset S1

<strong>Estrogen receptor alpha (ESR1) gene amplification is frequent in breast cancer. Subsequent Supplementary Material.</strong> Frederik Holst &amp; Christian Ruiz The supplementary material refers to: Holst, F., Stahl, P.R., Ruiz, C., et al. Estrogen receptor alpha (ESR1) gene amplification s frequent in breast cancer. Nature Genetics 39, 655-660 (2007). <strong>Subsequent Supplementary Figures S1-S7</strong> Illustration of the different Affymetrix 10K SNP array gene copy number data analysis methods and regarding segmented ESR1 copy number profiles of 22 breast cancers. Taken from Subsequent Supplementary Optical Dataset S1 a photographical documentation of <em>ESR1</em> FISH analysis signal appearance of tumors with <em>ESR1</em> amplification by SNP GeneChip analysis is added (figures 5A-C + 7A-C). DOI: http://dx.doi.org/10.6084/m9.figshare.1470035 <strong>Subsequent Supplementary Video Clips S1+S2</strong> The video clips document FISH analysis as performed for results documented in regarding subsequent supplementary figures 5A-C + 7A-C. Video clips are taken using an Olympus IX51 microscope at 100x magnification, an Olympus XM10 digital camera (1,376 x 1,032 pixels) and Olympus cellSens imaging software. DOI: http://dx.doi.org/10.6084/m9.figshare.1471677 <strong>Subsequent Supplementary Optical Dataset S1</strong> The optical dataset consists of 94 photographs documenting appearance of <em>ESR1</em> FISH analysis signals of 20 out of 22 tumors regarding SNP GeneChip analysis illustrated in subsequent supplementary figures S1-S3 and S4+S6. The 94 photographs are separated into 1-10 figures per tumor including up to three z-stacks per figure. Photographs are taken using an Olympus IX51 microscope at 100x magnification, an Olympus XM10 digital camera (1,376 ´ 1,032 pixels) and Olympus cellSens imaging software. DOI: http://dx.doi.org/10.6084/m9.figshare.1470130 <strong>Methods References</strong> Ruiz, C. Doctoral Thesis, Universität Basel (2006). Moelans, C.B., Holst, F., Hellwinkel, O., Simon, R. &amp; van Diest, P.J. ESR1 Amplification in Breast Cancer by Optimized RNase FISH: Frequent but Low-Level and Heterogeneous. PLoS One 8, e84189 (2013). Holst, F. FISH Analysis of ESR1 Amplification in Breast Cancer - A Video Illustration. DOI: http://dx.doi.org/10.6084/m9.figshare.1471765 (2015).