Role of Atg3, Atg5, and Atg12 in the crosstalk between apoptosis and autophagy in the posterior silk gland of Bombyx mori

Autophagy is a cellular mechanism that enhances cell survival in response to various stressors, including nutrient deprivation; however, it also plays a pivotal role in the regulation of programmed cell death. This study examined the effects of autophagy-related genes Atg3, Atg5, and Atg12 on apoptosis and autophagy during the degeneration of the posterior silk gland in Bombyx mori, employing RNA interference techniques. Apoptosis-specific markers and autophagic processes were evaluated in both control and treatment groups. The knockdown of all three genes resulted in a significant reduction in autophagy, modifications in the apoptosis process, aberrant expression of p53, and impaired lysosomal function. It was determined that Atg3 is involved in the regulation of intracellular mitochondrial homeostasis. Following the silencing of Atg5, evidence was obtained indicating the gene's role in regulating lysosomal pH. Notably, the loss of Atg3 and Atg5 was associated with an increase in apopt..., , , ## Role of Atg3, Atg5, and Atg12 in the crosstalk between apoptosis and autophagy in the posterior silk gland of Bombyx mori

[https://doi.org/10.5061/dryad.zkh1893kv](https://doi.org/10.5061/dryad.zkh1893kv)

## Description of the data and file structure

**File: RAW_DATA.xlsx**

**Description**:

* The **Figure 1A tab** within the Excel file presents the data derived from quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis conducted on both control and treatment groups following the silencing of the Atg3, Atg5, Atg6, and Atg12 genes in Bombyx mori.

- The tab labeled **Figure 2B tab** in the Excel file presents data analyzed through quantitative reverse transcription polymerase chain reaction (qRT-PCR) from both the control and gene-silencing groups. This analysis aims to assess the expression levels of autophagy-related genes, specifically Atg3, Atg5, Atg6, Atg8, and Atg12.

* In the Excel file, **Figure 3A tab** contains data on the spectrophotometric ana...,