Salvia sclarea Transcriptome or Gene expression

In this study, we constructed a normalised EST library from a calyx mRNA extract of Salvia sclarea. It was subjected to 454 pyrosequencing. The sequencing of one segment of a sequencing plate generated more than 190,000 EST sequences. After trimming small sequences less than 40 bp in length, 184,566 high quality ESTs with an average length of 214 bp totalling 39,5 Mb were conserved for further analyses. 158,322 ESTs were incorporated into 19,578 contigs leaving 26,244 singletons, for a total of 45,822 unique sequences covering 11 Mb. This study represents the first high throughput sequencing of cDNAs within Lamiaceae and for an angiosperm calyx. These sequences are a source of valuable genomic information in this important plant family.