Genome-wide maps of gene transcription level in BMDMs infected with recombinant Mycobacterium tuberculosis H37Ra strains.

We report the application of RNA-seq technology for high-throughput profiling of gene transcription in BMDMs. By obtaining over six billion bases of sequence from mRNA, we generated genome-wide gene transcription maps of BMDMs infected with recombinant Mycobacterium tuberculosis H37Ra strains. We find that a group of genes is significantly affected by MpbR. This finding indicates that MpbR regulates the host immune. Overall design: Examination of gene expression level in 3 H37Ra strains infected BMDM cells.