RNAseq analysis of genes differentially expressed in H1299 cells with anti-circadian rhythm dysfunctional treatment

We examined the biological difference between H1299 cells with the treatments targeting circadian rhythm in order to better understand circadian rhythm disruption as a feature of cancer. To this end, we knocked down CLOCK using siRNA (siCLOCK) or melatonin pre-treatment and assessed the gene expression pattern by RNA-Seq. Overall design: Nine samples obtained from H1299 cells including serum free medium (as control), siCLOCK and 0.1mM melatonin treatment group were analyzed. Three independent samples were analyzed for each group.