Comparison of PCR-free libraries
收藏NIAID Data Ecosystem2026-03-13 收录
下载链接：
https://www.ncbi.nlm.nih.gov/bioproject/PRJNA771676
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资源简介：
To enable comparision between NEBNext Ultra II, NEBNext Ultra II FS, and Illumina DNA PCR-Free library prep methods, sequencing libraries were prepared according the manufacturer recommendations from 250 ng of NA19240 cell line DNA and sequenced on a single NovaSeq 6000 S4 v1.5 flowcell (PE 2x150 bp). Reads were adapter trimmed (fastp 0.20.0), aligned) to the Telomere-to-Telomere chm13 reference (draft 1, bwa-mem 0.7.17), and duplicate marked (samblaster 0.1.24). 2.8B reads were randomly sampled from each bam (sambamba view -s 0.6.8).
创建时间：
2021-10-15
