Transcriptome analysis of xylose-fermenting yeast in industrial and laboratory fermentations

This project consists of the transcriptome analysis of a xylose-fermenting yeast, called Celere-2L(C2L), under industrial and laboratory conditions. Four fermentation time points were collected for each condition: (i) industrial (IN); (ii) hydrolyzed laboratory (LH) and (iii) synthetic laboratory (LS). Both biological samples are in triplicates. The industrial samples were collected in the BioFlex1 of the GranBio group, located in Sao Miguel dos Campos / State of Alagoas / Brazil. Laboratory scale fermentation was carried out using C2L yeast in two conditions: (1) hydrolyzed industrial lignocellulosic filtered through a 0.22 micron filter (called LH) and (2) synthetic medium containing YNB and similar concentrations of sugars (glucose and xylose), acids and inhibitors found in the industrial lignocellulosic hydrolyzate (called LS). All experiments were carried out for three complete fermentation cycles. For each cycle, the sampling points were selected according to yeast metabolism during fermentation to cover the main stages of fermentation kinetics: (1) predominantly glucose consumption, (2) transition between glucose/xylose consumption, (3) predominantly xylose consumption and (4) end of fermentation, thus totaling the four fermentation points. For industrial fermentation (IN) the points were extracted at 7h, 12h, 16h and 24h. For the hydrolyzed fermentation (LH), the fermentation points were extracted at 7h, 12h, 14h and 22h. Finally, for the synthetic fermentation (LS), the fermentation points were extracted at 6h, 11h, 14h and 18h.