Expression data from Ang II induced HUVECs. Expression data from Ang II induced HUVECs

lncRNAs were associated with the cardiovascular diseases since we used Affy lncRNA gene expression microarray to analyze differentially expressed lncRNAs in both normal and Ang II induced HUVECs Overall design: We incubated the HUVECs with Ang II of 1 μM for 48 h. Total RNA was harvested from HUVEC using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) and the RNeasy Kit (Qiagen Co., Hilden, Germany) according to the manufacturer's instructions, including a DNase digest ion step.To identify potential molecular factors associated with Ang II induced injury in HUVECs, we used Affy lncRNA gene expression microarray (Affymetrix company, USA) to analyze differentially expressed lncRNAs in both normal and Ang II induced HUVECs