Additional file 2: of Loss of DNA methylation is related to increased expression of miR-21 and miR-146b in papillary thyroid carcinoma

Table S1. Identification of 58 differentially expressed microRNAs detected in the comparison between PTC and NT from the TCGA database. Table S2. Integration between DNA methylation probes and the corresponding miRNA expression. Table S3. Differentially expressed genes in PTC compared to NT from TCGA database. Table S4. MicroRNA and target-mRNA interactions obtained by the integrative analysis (TCGA database), comprising the miRNAs potentially regulated by DNA methylation. Table S5. Comparison of miRNA genes methylation, miRNA, and target gene expression with clinical features (clinical stage, extrathyroidal extension, node metastasis and BRAF mutation) using the TCGA database. Table S6. List of 452 target transcripts of miRNA potentially regulated by methylation after mimic transfection in TPC1 cell line. Table S7. Significant pathways (P value< 0.05) enriched by target genes of hsa-miR-21-5p and hsa-miR-146b (5p and 3p) identified by IPA. Table S8. Significant pathways (P value< 0.01) enriched by target genes of hsa-miR-21-5p and hsa-miR-146b (5p and 3p) identified by KOBAS 3.0 ( http://kobas.cbi.pku.edu.cn ). Table S9. Clinical-pathological characteristics of the patients included in the study. Table S10. Primer sequences, amplicon size, number of CpGs flanked in the amplification and PCR temperature conditions. Table S11. Selection criteria of the miRNA targets selected for RT-qPCR validation. Table S12. Characteristics of the primers used in the transcripts expression level analyses by RT-qPCR. (XLSX 295 kb)