Background information and MIC data of the used samples, and results of the developed MAMA tests.

A and B: Background information of the 92 M. synoviae strains provided by previous studies [9; 12] and results of fluoroquinolone (A), macrolide and lincomycin (B) susceptibility determination by broth microdilution and the developed melt-MAMA tests. C and D: Background information of the 20 clinical specimens and 20 clinical sample-derived M. synoviae isolates examined in this study and results of fluoroquinolone (C), macrolide and lincomycin (D) susceptibility determination by broth microdilution and the developed melt-MAMA tests. MIC values were considered to be elevated above 1.25 μg/ml for enrofloxacin and difloxacin; 8 μg/ml for tilmicosin; 1 μg/ml for tylosin; 0.5 μg/ml for tylvalosin; and 2 μg/ml for lincomycin. Color codes indicating: elevated MIC (dark grey); low MIC (light grey); genotype assignment is congruent with the MIC data (green); genotype assignment is incongruent with the MIC data, i.e. false result (yellow); congruency cannot be evaluated due to that the sample was below detection limit in some of the assays (blue); excluded from the evaluation due to missing MIC data (no color). PCR: polymerase chain reaction; CT: cycle threshold; MAMA: mismatch amplification mutation assay; MIC: minimal inhibitory concentration; Gt: genotype according to whole genome sequence; Tm: melting temperature; n.t.: not tested; n.a.: not available; L: genotype characterized by low MIC values; H: genotype characterized by high MIC values; Het: heterozygous genotype; NS: non-specific melting temperature; -: undetected; *: expected to be undetectable by the assay
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