Transcriptional profiling of Cd169-lineage lymph node macrophages

Tingible body macrophages (TBMs) exist in lymph node B cell follicles and are responsible for clearing apoptotic B cells. Our data suggests that TBMs are of Cd169-lineage. We isolated cells from Cd169Cre/+.Tdtomatofl/fl reporter mice and transcriptionally profiled them to identify putative TBMs and chacterise their development and function relative to other LN cells of the Cd169 lineage. Overall design: Cd169Cre/+.Tdtomatofl/fl mice were immunised with hen egg lysozyme (HEL). Lymph nodes were harvested from 2 unimmunised and 2 immunised mice 9 days later. The cells were stained with CITE-seq antibodies and hashtags, and Tomato pos CD11b hi cells were isolated by Fluorescence-activated cell sorting (FACS). As the cells were hashtagged, all samples were combined and split cross two 10X capture reactions