Effect of knockdown of RKIP(=PEBP1) on gene expression in mouse breast cancer cells

Raf-1 kinase inhibitor protein (RKIP = PEBP1) has been shown to negatively regulate signaling pathways including the MAPK and NFkappaB pathways involved in cancer progression and metastasis. RKIP acts as a suppressor of metastasis, but the exact mechanisms are unclear. In this study, knockdown of RKIP expression was used to identify the targets of RKIP expression. GSEA analysis identified interferon response genes as downregulated in the RKIP knockdown cells. The mouse breast cancer cell line 168FARN was transfected with expression vectors for either RKIP or control luciferase siRNAs. Total RNA was extracted from biological triplicates and hybridized to Affymetrix Mouse Gene 2.0 ST microarray chips.