Additional file 5: of Comparative analysis of single-stranded DNA donors to generate conditional null mouse alleles

Figure S2. A, B. Frequency distributions of (A) projects with conditional, null, or both allele types, and (B) projects with loxP in cis, in trans, or both, binned by loxP distance. Project results derived from genotyping information gathered from F0 (A) and N1 (B) mice. LoxP distance calculated from genomic distance between Cas9 cut site of each sgRNA. X-axis labels indicate the median value of each bin. (A) Left Y-axis depicts the number of animals screened for each loxP distance bin, while the right Y-axis depicts the allele percentage within each loxP distance bin. (B) Left Y-axis depicts the number of founders bred for each loxP distance bin, while the right Y-axis depicts the transmission percentage for all projects within each loxP distance bin. (XLSX 254 kb)