Data from: Chromosome-level reference genome of the beach false foxglove, Agalinis fasciculata (Orobanchaceae).

This repository contains the data for the reference genome of Agalinis fasiculata. The code is available on GitHub. If what you’re looking for is not available here or on GitHub, feel free to contact me via email at [e-mail] or [e-mail]. AbstractOrobanchaceae is the largest family of parasitic plants, encompassing a full spectrum of parasitic strategies, ranging from autotrophic to holoparasitic. Agalinis is a genus of facultative hemiparasites comprising about 70 species distributed throughout the Americas, including several endemic and rare taxa. Agalinis fasciculata, the beach false foxglove, is a widely distributed species across southeastern North America. Here, we use PacBio HiFi, Omni-C, and RNA-seq data to generate the first high-quality reference genome for the genus. The nuclear genome is 2.29 Gb in size, with most sequences anchored to 14 pseudochromosomes and an N50 of 162 Mb. BUSCO analyses indicate high completeness (98.4%). Structural genome annotation identified 34,133 protein-coding genes and 39,266 transcripts, most of which have at least one functional annotation. The plastid and mitochondrial genomes were also assembled. We further examined genetic diversity and demographic history in A. fasciculata, revealing low genome-wide heterozygosity and evidence of inbreeding. This reference genome is an important genomic resource for understanding the evolutionary history of the genus and the evolutionary patterns of parasitism within Orobanchaceae. Pedro H Pezzi, Maribeth Latvis, Chromosome-level reference genome of the beach false foxglove, Agalinis fasciculata (Orobanchaceae), Genome Biology and Evolution, 2026; evag116, https://doi.org/10.1093/gbe/evag116