Chemtax version 1.95 for calculating the taxonomic composition of phytoplankton populations

CHEMTAX V1.95This program is based on CHEMTAX V1 which was produced by the Commonwealth Scientific and Industrial Research Organisation (CSIRO), Division of Marine Research, GPO Box 1538, Hobart, Tasmania 7001, Australia (Copyright CSIRO). The MATLAB script for the original program was written by Mark Mackey (CSIRO), in association with Dennis Mackey (CSIRO), Harry Higgins (CSIRO) and Simon Wright (Australian Antarctic Division, AAD, 203 Channel Highway, Kingston, Tasmania, Australia). It was published in: Mackey, M.D., Mackey, D.J., Higgins, H.W., and Wright, S.W. (1996). CHEMTAX - a program for estimating class abundances from chemical markers: application to HPLC measurements of phytoplankton. Marine Ecology Progress Series 144: 265-283, doi: 10.3354/meps144265The current version (CHEMTAX V1.95) was written by Chris Boucher (Barking Spider P/L), in association with Simon Wright and Harry Higgins (AAD). Peripheral software was written by Simon Wright (AAD).It is a stand-alone program that takes input from a Microsoft Excel worksheet.It calculates the taxonomic composition of phytoplankton populations based on pigment data and a table of the expected taxonomic composition and pigment:chl a ratios entered by the operator.The zip folder contains Chemtax.exe, Chemtax2.dll, Testrun195.xls and CHEMTAXv195Helper7.xlm.Also included are two Word files (Chemtax 195 Instructions.doc, and Chaxmanw.rtf, which is the manual for Version 1).The latter manual contains details on the algorithms used in CHEMTAX V1, which are unchanged, but the operating instructions in that manual are superseded by those in Chemtax 195 Instructions.doc.Please note: CHEMTAX must not be used as a black box. It will not deduce what taxa are in the water. The user must input the expected taxa and their expected pigment composition, then CHEMTAX will calculate the contributions of each taxon to the total in each sample. It is imperative that the user understands the function of CHEMTAX, and the taxonomic distribution of pigments (including the potential ambiguities) if useful data are to be obtained.A detailed strategy for applying CHEMTAX (and interpreting pigment data in general) is given in Higgins et al (2011). An example of combining CHEMTAX with other data is given in Wright et al (2010).Higgins H.W., Wright S. W., Schluter L. (2011). Quantitative Interpretation of Chemotaxonomic Pigment Data, Chapter 6, Phytoplankton Pigments: Characterization, Chemotaxonomy and Applications in Oceanography, Suzanne Roy, Einar Skarstad Egeland, Geir Johnsen and Carole Anne Llewellyn (eds.) Cambridge University Press.Wright, SW, van den Enden, RL, Pearce, I, Davidson, AT, Scott FJ, Westwood, KJ (2010). Phytoplankton community structure and stocks in the Southern Ocean (30 - 80 degrees E) determined by CHEMTAX analysis of HPLC pigment signatures. Deep-Sea Research II 57, 758-778Registration:After downloading the files, please email the enclosed registration form to [e-mail] with CHEMTAX in the title

CHEMTAX V1.95
本程序基于澳大利亚联邦科学与工业研究组织（Commonwealth Scientific and Industrial Research Organisation, CSIRO）海洋研究分部开发的CHEMTAX V1版本，该分部地址为澳大利亚塔斯马尼亚州霍巴特市GPO信箱1538号，邮编7001（版权归CSIRO所有）。原始程序的MATLAB脚本由Mark Mackey（CSIRO）编写，合作作者包括Dennis Mackey（CSIRO）、Harry Higgins（CSIRO）以及Simon Wright（澳大利亚南极局，Australian Antarctic Division, AAD，地址：澳大利亚塔斯马尼亚州金斯顿市海峡公路203号）。该程序发表于：Mackey, M.D., Mackey, D.J., Higgins, H.W., 与Wright, S.W. (1996). 《CHEMTAX——基于化学标志物估算类群丰度的程序：在浮游植物高效液相色谱（High Performance Liquid Chromatography, HPLC）检测数据中的应用》，《海洋生态学进展系列》（Marine Ecology Progress Series）144卷：265-283，doi: 10.3354/meps144265。

当前版本（CHEMTAX V1.95）由Chris Boucher（Barking Spider P/L）编写，合作作者为Simon Wright与Harry Higgins（澳大利亚南极局）。辅助软件由Simon Wright（澳大利亚南极局）开发。

本程序为独立软件，可读取Microsoft Excel工作表中的输入数据，基于色素数据、操作人员输入的预期分类组成表以及色素与叶绿素a（chlorophyll a, chl a）的比值表，计算浮游植物种群的分类组成。

该压缩包包含Chemtax.exe、Chemtax2.dll、Testrun195.xls以及CHEMTAXv195Helper7.xlm。此外还包含两份文档：Chemtax 195 Instructions.doc（CHEMTAX 195操作指南）以及Chaxmanw.rtf（即版本1的操作手册）。后者包含了CHEMTAX V1所使用算法的详细信息，该算法未作修改，但该手册中的操作说明已被Chemtax 195 Instructions.doc中的内容取代。

请注意：CHEMTAX不可作为黑箱工具使用。其无法自动推断水体中存在的分类群。操作人员必须输入预期的分类群及其预期的色素组成，随后CHEMTAX将计算每个样本中各分类群对总生物量的贡献。若要获得有效数据，操作人员必须理解CHEMTAX的工作原理以及色素的分类分布（包括潜在的歧义性）。

关于应用CHEMTAX（以及一般性色素数据解读）的详细策略，可参见Higgins等人（2011）的研究。将CHEMTAX与其他数据结合使用的示例可见于Wright等人（2010）的研究。
Higgins H.W., Wright S. W., Schluter L. (2011). 《化学分类色素数据的定量解读》，收录于《浮游植物色素：表征、化学分类学及海洋学应用》（Suzanne Roy、Einar Skarstad Egeland、Geir Johnsen与Carole Anne Llewellyn编辑），剑桥大学出版社。
Wright, SW, van den Enden, RL, Pearce, I, Davidson, AT, Scott FJ, Westwood, KJ (2010). 《南大洋（东经30°-80°）浮游植物群落结构与生物量：基于HPLC色素信号的CHEMTAX分析》，《深海研究第二辑》（Deep-Sea Research II）57卷：758-778。

注册须知：
下载文件后，请将附带的注册表以主题为"CHEMTAX"的邮件发送至[e-mail]。

搜集汇总

背景与挑战

背景概述

Chemtax version 1.95是一个用于计算浮游植物种群分类组成的软件工具，基于MATLAB脚本开发，适用于全球海洋和极地地区的浮游植物研究。用户需输入预期的分类组成和色素数据，软件将计算各分类群在样本中的贡献比例。