Functional reprogramming of neutrophils within tumors by hypoxia-driven histone lactylation [scRNA-seq]

We used scRNAseq to study the mechanism underlying the functional reprogramming in brain tumors. scRNA-seq of FACS-sorted neutrophils was performed on mice with brain tumors (SB28). We showd that the immunosuppressive ability of brain-tumor infiltrating neutrophils was exclusively mediated by a population of highly glycolytic neutrophils expressing the transferrin receptor CD71. We FACS-sorted neutrophils (CD45+ CD11b+ LY6Ghigh LY6Clo/-) from blood and tumors of mice with brain tumors (SB28) at late stage (day 18) and processed them for sc-RNAseq using the 10x Genomics Chromium platform.