NCMD assembly

For genome assembly, we generated short and long reads using the DNA sample of an adult male of Nanchukmacdon. Then, we constructed a genome assembly using the sequencing reads with the reference-guided approach. The 80.14x raw PacBio subreads were assembled and polished to generate 1,942 high-quality contigs supported by at least 50 PacBio subreads. To generate a chromosome-level assembly, the high-quality polished contigs were then further assembled by an improved version of RACA that can utilize both the genome information of related species and diverse types of sequencing data. The assembly was used to build the final assembly after one more polishing step using short reads. The sequencing read data can be obtained at NCBI SRA under the project of PRJNA967127