Liver and plasma chemistries of ADKD WT mice at 10 days p.t.

Mice were chow-fed, 10-week-old B6 (WT) males, intravenously injected with ADKD vectors expressing RNAi for lacZ or Ildr2. Measurements were taken at 10 days p.t. (following either a 24-hr fast “Fasted” or following a 24-hr fast and 12-hr refeeding “Refed”). n = number of animals in each study. n/a: not applicable. Data shown are mean ± SEM; P values were calculated by 2-tailed t test. TG, triglycerides; TCH, total cholesterol; FFA, free fatty acids; ALT, alanine aminotransferase; AST, aspartate aminotransferase; HOMA2-IR, homeostasis model assessment-estimated insulin resistance; HOMA-2-B (%), homeostasis model assessment-β- cell function. In contemporaneous experiments in our laboratory, B6 mice fasted for 12 hr and not treated with adenovirus had plasma ALT of 86.0 mU/L and AST of 94.6 mU/L.