Study of differential chromatin accessibility within primary breast epithelial cells from healthy donor and its TONSL overexpressing counterpart. Study of differential chromatin accessibility within primary breast epithelial cells from healthy donor and its TONSL overexpressing counterpart

To evaluate the probable role of TONSL during tumorigenesis we overexpressed TONSL in primary breast epithelial cells and studied differential chromatin accessibility. Overall design: KTB103 primary and TONSL overexpressing KTB103 cells were subjected to ATAC-seq using the previously established protocol (Oncogene 40:1332-1346) . Assays were done in biologic triplicates with ~50,000 cells.