Rat spinal cord (basal, post TLR2 and TLR4 agonist stimulation) was positively correlated with periphery outputs.

IL-1β level released from (A) basal spinal cord supernatant (P = 0.00048, adjusted R-square = 0.56) (B) basal spinal cord (P = 0.011, adjusted R-square = 0.47) (C) spinal cord supernatant (P = 0.01, adjusted R-square = 0.52) (D) spinal cord post Pam3CSK4 (P = 0.04, adjusted R-square = 0.29) (TLR2) stimulation at 100 µg/mL (E) spinal cord supernatant (P = 0.02, adjusted R-square = 0.48) (F) spinal cord (P = 0.09, adjusted R-square = 0.26) post LPS (TLR4) stimulation at 100 µg/mL was found to be significantly correlated with the estimated values predicted from peripheral tissue outputs in rats from Neuronal and subcutaneous. Pearson correlation was used and data shown in panel (A, E and F) have been log transformed and linear regression with 95% confidence interval curves are shown on the graph.