Chronic TCR signaling-driven suppression of FOXO1-KLHL6 axis promotes T cell exhaustion

This study was published as a “Brief Report” and therefore does not include an abstract. Overall design: Naive CD8? T cells were isolated from the spleens of OT-I mice and activated with anti CD3 (2ug/ml) and anti-CD28 (1ug/ml) antibodies in the presence of IL-2 (10ng/ml) for 48 h. The cells were then removed from the activation plates and cultured under normal conditions. CD8? T cells were collected at the naive state and at 24 h, 48 h, and 72 h after activation for RNA-seq analysis, with three biological replicates per group.