Statistical analysis of differences in methylation levels in different parts of the gene body.

Single-factor ANOVA was used to identify significant differences between the progeny of plants exposed to 25 mM NaCl and the progeny of control plants (25mM_ct), between the progeny of plants exposed to 75 mM NaCl and the progeny of control plants (75mM_ct), and between the progeny of plants exposed to 75 mM NaCl and the progeny of plants exposed to 25 mM NACl (75mM_25mM). The analysis was performed for the entire sequence of the gene body as well as for the 5′/3′ ends, the middle part, exon/intron regions.