Flow cytometry dataset of dexamethasone and immune checkpoint blockade in 4T1 breast cancer model (Intracellular cytokine staining)

Flow cytometry data were generated using the 4T1 breast cancer model to evaluate the effects of dexamethasone alone or in combination with immune checkpoint blockade (ICB) on tumor-infiltrating lymphocytes. Intracellular cytokine staining (ICS) was performed to assess IFNγ, TNFα, and Granzyme B production by T cells following stimulation. These experiments aimed to determine whether dexamethasone enhances the ICB-induced functional activity of tumor-infiltrating T cells. Tumor-infiltrating cells were stained with Zombie Yellow for viability, FITC-conjugated CD3, a dump channel (PE-CF594 F4/80, Ly6G, B220), PerCP-Cy5.5–CD4, APC/Cy7–CD8, Pacific Blue–CD45, PE–TNFα, PE/Cy7–IFNγ, and APC–Granzyme B.