The formation of chimeric sequences can create significant methodological bias in PCR-based DNA metabarcoding analyses. During mixed-template amplification of barcoding regions, chimera formation is f
This study focuses on measuring the rate of chimera formation in Illumina sequencing and how it compares to the detection of human-bacteria read pairs that are putative lateral gene transfers.
*Values are averages ± STDEV calculated from multiple replicates of MC, and from replicates of multiple clinical samples originating from different body sites.
These bash commands were used to search for and remove any read containing the EcoRI and MspI recognition sites, which are potential chimeric sites formed during ligation.
A fundamental question in developmental biology is whether there are mechanisms to detect stem cells with mutations that, although not adversely affecting viability, would compromise their ability to
