Short EdU pulse in <i>Nematostella</i> during starvation and refeeding (Fig. 4IJ)

Per sample a group of 10 juvenile polyps were labeled for 60min with EdU (300uM, 2%DMSO) over several time points after feeding (1 day - 2d - 5d- 12d - 21d as well as 20d starvation + 1 day post refeeding) and then dissociated using ACME. Cells were stained with 1μg/ml FxCycle violet DNA dye (Invitrogen) and EdU was labelled with Alexa488 using a Click-it reaction.Flow cytometry was performed a BD LSRFortessa (BD Life Sciences) and the EdU positive cells in samples were determined based on Alexa488 fluorescence in comparison with the DMSO controls on cells within the cell cycle (based on DNA staining) using FlowJoV10.8 (BD Life Sciences)..wsp files contain the samples, gating strategy and cell populations used for the analysis in FlowJo.fcs files represent the individual flow cytometry output files that were analysed in the .wsp file<br>