ubH2B CHIP-seq in Wild Type and Wac Conditional Knockout Activated B Cells

To investigate the function of Wac in regulatng H2B ubiquitination during B cell activation and differentiation, we used Cre-Loxp system to generate Wac conditional knockout mice (Wacf/f Cd19cre/+) and performed ubH2B chromatin immunoprecipitation coupled with high-throughput sequencing (Chip-seq) in Wac KO and WT LPS activated B cells. Overall design: Splenic B cells from wild-type and Wac Cd19-cre conditional knockout mice were purified and cultured 24 hours with 20 ug/mL LPS. Then analyzed using ubH2B Chip-seq.