Mark S. Ladinsky, Christine C. Wu, Shane McIntosh, J. Richard McIntosh, Kathryn E. Howell (2011) CIL:24516, Rattus norvegicus, epithelial cell. CIL. Dataset

This image is one from a set of 40nm thick serial sections through part of a Golgi ribbon from a normal rat kidney cell, generated using transmission electron microscopy following incubation at 15°C to block transport out of the Golgi complex. Following temperature block, large bulging domains appear on the three trans-most cisternae. The images in this set were used in 3-dimensional reconstructions of the Golgi apparatus. For more information see: Ladinsky et al. (2002) Structure of the Golgi and distribution of reporter molecules at 20°C reveals the complexity of the exit compartments, Mol Biol Cell 13:2810-2825. The other images from this serial set are included in this image group.

本图像系从正常大鼠肾脏细胞高尔基体带部分获取的40纳米厚连续切片集之一，采用透射电子显微镜在15°C条件下进行孵育以阻断高尔基体复合物中的物质运输。温度阻断后，在三个最外层囊泡上出现显著膨胀的域。本组图像被用于高尔基体的三维重建。更多详细信息请参阅：Ladinsky等（2002）在20°C下高尔基体结构与报告分子分布的研究揭示了出口区室的复杂性，Mol Biol Cell 13:2810-2825。本连续切片集中的其他图像亦包含在本图像组中。