Hans Ris (2010) CIL:9528, notopthalmus viridescens, erythrocyte. CIL. Dataset

Newt (Notopthalmus viridescens) erythrocytes were isolated in 0.1M KCl, spread on the surface of 5mM Na-citrate, picked up on formvar-carbon films, fixed with glutaraldehyde and paraformaldehyde and negatively stained with sodium tetraborate. Images were recorded at 40KX and accelerating voltage of 1MeV at the University of Wisconsin HVEM facility.This image was taken with a specimen tilt of 45 degrees. A grouped image of the same field at a 55 degree tilt provides a stereo view of the tangle of chromatin fibers emanating from the dispersed nucleus.

来自新特（Notopthalmus viridescens）的红细胞在0.1M氯化钾溶液中分离，随后铺展于5mM柠檬酸钠溶液表面，经形式碳膜吸附后，以戊二醛和过氧化甲醛进行固定，并使用四硼酸钠进行负染色。图像在威斯康星大学HVEM设施中以40KX放大倍数和1MeV加速电压下记录。本图像拍摄时样本倾斜角度为45度。同一视野下以55度倾斜拍摄的分组图像，提供了来自分散核的染色质纤维错综复杂的立体视角。