Mosquito salivary sialokinin reduces human primary monocyte activation

Mosquito saliva contains bioactive factors that enhance viral infection, with sialokinin identified as a key contributor to vascular leakage and viral spread in mice. Understanding effect of sialokinin on human moncoytes, the most important innate cells that responsible for the viral-induced inflammatory, will provide important insight to host-virus-vector interaction in arbovirus infection. Freshly purified human monocytes (1,000,000 cells/well) were incubated with or without sialokinin peptides in complete IMDM supplemented with 10% (vol/vol) HS (Sigma-Aldrich) and 1% (vol/vol) penicillin-streptomycin. Untreated monocytes served as no-treatment controls. Total RNA was isolated from primary human monocytes using a RNeasy Micro Kit (Qiagen), following the manufacturer's instructions.