Retinoic Acid Receptor Gamma is Required for Proliferation of Pancreatic cancer cells

Despite of the expectation that retinoic acid receptor could be the potential therapeutic targets for pancreatic cancers, there has been the lack of information about the role and the impact of Retinoic acid receptor gamma (RARγ, RARG) on pancreatic cancer, unlike other two RARs.RARG is commonly over-expressed in human pancreatic cancer and is an independent diagnostic marker predicting the poor prognosis of pancreatic cancer patients. In addition, we demonstrated that the reduction in the expression of RARG in human pancreatic cancer cells dramatically suppress their proliferation and tumor growth in vivo, partially attributable to the down-regulation of tumor-supporting biological processes such as glucose transport and the decreased expression of various oncogenes like MYC and STAT3. Crispr-cas9 was used to construct RARG-Knockout PANC1 cells lines. Transcriptome analysis was performed in wildtype and RARG-Knockout PANC1.