Methylomic analysis identifies the involvement of migration and adhesion genes in the ageing of primary haematopoietic stem cells

we utilize Nano-MeDIP-seq for the analysis of the LT-HSC methylome and, for the first time, simultaneously interrogate the methylome and transcriptome of a homogeneous population of primary murine HSCs, in order to define the underlying causes of changes in HSC functionality during normal ageing. We isolated and phenotyped primary LT-HSCs from young, middle-aged and old mice and subjected them to comprehensive methylome (MeDIP-seq) and transcriptome (RNA-seq) analysis.本研究采用纳米甲基化DNA免疫沉淀测序（Nano-MeDIP-seq）分析长期造血干细胞（LT-HSC）的甲基化组，并首次同时对均一的小鼠原代造血干细胞群的甲基化组与转录组进行解析，以期阐明正常衰老过程中造血干细胞功能改变的潜在成因。我们从年轻、中年及老年小鼠体内分离原代长期造血干细胞并完成表型鉴定，随后对其开展全面的甲基化组（MeDIP-seq，甲基化DNA免疫沉淀测序）与转录组（RNA-seq，RNA测序）分析。