mRNA expression profile in LGP2 and LGP2-4KR transgenic (Tg) HEK293 cells stimulated with short poly I:C

mRNA expression profiles were compared between wild-type LGP2 and four amino acid replacement LGP2 (LGP2-4KR) cells in the presence or absence of a RIG-I ligand, short poly I:C. LGP2 and LGP2-4KR Tg HEK293 cells were cultured in a 24-well-plate, and were then stimulated with or without 100 ng/ml of short poly I:C for 12 hours. Total RNA was extracted from stimulated and unstimulated HEK293 cells, and was subjected to the microarray analysis.