rhGH/ hGHR Binding energy predicted by HomologyScanner and SSIPe, and kinetics of binding measured by Surface Plasmon Resonance, ordered by koff.

Green shading indicates KD comparable to that of WT. ΔΔGexperimental.was computed based on the measured KD and T = 273K. In these cases, kon is also slower than WT, such that the only E174A has KD lower than that of WT. The Pearson correlation between ΔΔGhomologyScanner and ΔΔGexperimental was -0,18, and that between ΔΔGSSIPe and ΔΔGexperimental was -0,40. This had no particular significance, with p-values of 0,4 and 0,07, respectively, by two-tailed t-test.