Defining the role of the General Stress Response regulators, SigT and GsrN, in control of the hyperosmotic stress response in Caulobacter crescentus

We report gene expression responses of Caulobacter crescentus to osmotic upshock (150 mM sucrose). The goal of this study is compare the transcriptional responses of wild type, sigT deletion, and gsrN overexpression (gsrN++) strains. The data provide a high resolution view of how two major regulators of the general stress response (sigT and gsrN) shape transcription upon shift to a hyperosmotic environment. Caulobacter crescentus wild type, ΔsigT and gsrN++ strains were grown to mid log phase. Cultures were split, and either treated with 150 mM sucrose (to induce hyperosmotic shock) or untreated. After treatment, cells were rapidly pelleted and resuspended in Trizol for RNA purification.