Shared roles of immune and stromal cells in the pathogenesis of human bronchiolitis obliterans syndrome

We performed single cell RNA sequencing using the 10X genomics platform on explanted human lungs from 4 CLAD-BOS patients, 3 cGVHD-BOS patients, and 3 deceased controls to identify cell types, genes, and pathways enriched in BOS to better understand disease mechanisms We analyzed distal lung specimens from patients undergoing first lung transplant for cGVHD-BOS, second lung transplant for CLAD-BOS, and healthy controls (NC) using the 10X Genomics Chromium Controller platform with Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1. Sequencing was performed on an Illumina Novaseq 6000 instrument. We compared T-cell, macrophage, epithelial, and stromal transcriptomes between 1) CLAD-BOS and NC 2) cGVHD-BOS and NC 3) CLAD-BOS and cGVHD-BOS *************************************************************** Raw files for human/patient samples were not submitted to GEO due to concerns about submitting personally identifiable sequence data for open access. ***************************************************************