Colony PCR screen of clones created with primer extension mutagenesis of CDR-H3 loop.
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Analysis I: colonies from cm-plates (all grow) amplified with WO375 & B1 (all amplified) and digested with SacII. Analysis II: colonies from cm & amp-plates (mutants grow) amplified with A1 & pAK400rev (wild type specific). K: Kunkel, U: UDG, R: RCA, cm: chloramphenicol and amp: ampicillin.
创建时间：
2015-12-02
