Next Generation Sequencing of first trimester human cytotrophoblasts expressing a constitutively active mutant (5SA) of the transcriptional co-activator YAP.

Aim of the study was to elucidate YAP-regulated trophoblast-specific genes by analyzing differentially-expressed gene signatures between first trimester human cytotrophoblasts (vCTBs) transfected with a YAP-5SA mutant or a control plasmid. To examine gene expression patterns of human trophoblasts expressing constitutively active YAP 5SA Next Generation Sequencing was performed. vCTBs, isolated from pooled placentas from 6-8th weeks of gestation, were transfected with pCMV-flag YAP2 -5SA or as control with pcDNA 3.1(-)plasmid DNA using electroporation following 24h cultivation on fibronectin-coated cell culture dishes. The analyses comprise in total 6 samples, 3 replicates of 3 individual trophoblast cell preparations.