Inducible meiotic cDNA libraries for genome-wide studies of gene isoforms

We report the construction of 5 yeast meiotic cDNA libraries and perform proof-of-principle screens to show that these yeast cDNA libraries can be used to identify genes and gene isoforms that are important for competitive fitness. Samples 1-25 are from different stages of cDNA library construction and deep sequencing was used to characterize gene representation in each yeast cDNA library. Samples 26-175 are from proof-of-principle competitive fitness screens.本研究构建了5个酵母减数分裂互补DNA（cDNA）文库，并开展了原理验证筛选实验，证实该类酵母互补DNA文库可用于鉴定对竞争适合度具有关键作用的基因及基因异构体。其中，样本1至25取自互补DNA文库构建的不同阶段，本研究采用深度测序技术对每个酵母互补DNA文库中的基因分布情况进行了表征分析；样本26至175则来自原理验证型竞争适合度筛选实验。