conserved-primer-candidates-for-orthologous-segments
收藏DataONE2013-08-19 更新2024-06-27 收录
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资源简介：
A spreadsheet containing sets of forward and reverse PCR primers in successive rows, for each of the 56 segments in 3-way-aligned-orthologous-segments.fas. The primers were predicted with BatchPrimer3 (You et al. 2008) and the principal output such as expected product size and Tm is included. We also include the estimated dN/dS ratio for each pairwise comparison of the three frog transcriptomes, as an index of the overall conservation of each set of predicted cDNAs. The primer sets have not been systematically evaluated on either cDNA or genomic DNA, and may or may not bridge intronic sequence.
创建时间：
2013-08-19
