Near telomere-to-telomere genome assembly of the Beagle dog (Canis lupus familiaris)

The Beagle dog (Canis lupus familiaris) plays a dual role as both a beloved family pet and an invaluable research model animal. Here, we present the first telomere-to-telomere genome assembly of the Beagle dog. The genome assembly size is 2.46 Gb with a contig N50 of 63.17 Mb, and the largest contig spans 123.45 Mb. The BUSCO completeness is 98.30%. A total of 99.92% of the contigs were anchored to 38 acrocentric autosomes and a metacentric sex chromosome. Only 13 gaps remained to be resolved, and 29 out of the 39 chromosomes are telomere-to-telomere gap-free. Two hundred and seven out of the total 447 rRNA genes were enriched on chromosome 4. Repeats comprised 34.90% of the genome, which is higher than that of other canine genomes. A total of 21,146 protein-coding genes were predicted, and 99.68% of them were functionally annotated.