Nociceptor neurons affect cancer immunosurveillance [RNA-seq 1]

Neuron, melanoma and CD8 co-cultures Naive splenocyte CD8+ T-cells were FACS-purified from RAMP1wt (CD45.1+) or RAMP1-/- (CD45.2+) mice, cultured and amplified. 8-week-old female RAG1-/- mice were transplanted (i.v., 2.5x106 cells) with either of RAMP1-/- or RAMP1wt CD8+ T-cells or 1:1 mix of 5x106 RAMP1-/- and RAMP1wt CD8+ T-cells. One week post transplantation, the mice were inoculated with B16F10-mCherry-OVA cells (5x105 cells; i.d.). On day 14, tumor and draining lymph node were harvested, and RAMP1-/- (CD45.2+) and RAMP1wt (CD45.1+) CD8+ T-cells were FACS-purified and RNA sequenced.