soil metagenome Targeted Locus (Loci)

We used soil from a 33-year fertilization experiment and performed high-throughput pyrosequencing analyses targeting the ammonia monooxygenase alpha subunit (amoA) gene to determine whether and how the composition and structure of ammonia-oxidizing microbes in different particle-size fractions (>2000, 2000–200, 200–63, 63–2 and 2–0.1µm) have changed in response to chronic inorganic (NPK) and organic (NPKM) fertilizer additions under a rice-wheat rotation.