RT-PCR analysis of the human dopamine and norepinephrine transporter variants produced by alternative splicing.

A. Total RNA from white blood cells of three healthy volunteers (lane 1–3) was used to synthesize first strand cDNA for analyzing the expression of a variant of the dopamine transporter by RT-PCR and agarose gel electrophoresis. B. Expression of variants of the norepinephrine transporter was examined in adult whole brain (lane 1), fetal whole brain (lane 2), placenta (lane 3) and SK-N-SH cells (lane 4) with controls (FL hNET, lane 5 and hNETΔEX6, lane 6). The data represent a typical example from one experiment followed by at least 2 additional experiments with similar results. M; DNA marker of 100 bp ladders.